Liposomes, Part D by Nejat Duzgunes

By Nejat Duzgunes

Liposomes are mobile buildings made from lipid molecules. very important as a mobile version within the research of simple biology, liposomes also are utilized in medical functions equivalent to drug supply and virus experiences. Liposomes half D is a continuation of past MIE Liposome volumes A, B, and C. Contents: Antibody or Ligand specific Liposomes; setting delicate liposomes; liposomal oligonucleotides; liposomes in vivo

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Liposomes, Part D

Liposomes are mobile constructions made of lipid molecules. very important as a mobile version within the research of easy biology, liposomes also are utilized in scientific purposes reminiscent of drug supply and virus reports. Liposomes half D is a continuation of prior MIE Liposome volumes A, B, and C. Contents: Antibody or Ligand exact Liposomes; atmosphere delicate liposomes; liposomal oligonucleotides; liposomes in vivo

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Holt, Michael L. Johnson, and Gary K. Ackers Volume 380. Energetics of Biological Macromolecules (Part E) Edited by Jo M. Holt, Michael L. Johnson, and Gary K. Ackers Volume 381. Oxygen Sensing Edited by Chandan K. Sen and Gregg L. Semenza Volume 382. Quinones and Quinone Enzymes (Part B) Edited by Helmut Sies and Lester Packer Volume 383. Numerical Computer Methods (Part D) Edited by Ludwig Brand and Michael L. Johnson Volume 384. Numerical Computer Methods (Part E) Edited by Ludwig Brand and Michael L.

5 mol% maleimido-PEG-PE, a high proportion of the Fab0 fragments that bear free sulfhydryl groups can become liposome coupled under the conditions described earlier. An additional advantage of maleimide-functionalized PEG-PE ‘‘anchors’’ is that the PEG linker allows better exposure of the liposome-coupled Fab0 to its target ligand, with less steric hindrance from the liposome surface and greater flexibility of attachment. An illustration of the use of liposome-coupled Fab0 fragments to promote liposome uptake by mammalian cells is shown in Fig.

Triton X-100, 20% (w/v) in coupling buffer. Procedure Immediately after the calcein-loaded liposomes are eluted from the Sephadex G-75 column, the fluorescence (F) of duplicate aliquots is 22 R. Bredehorst, F. S. Ligler, A. W. Kusterbeck, E. L. Chang, B. P. -W. Vogel, Biochemistry 25, 5693 (1986). 23 T. M. Allen and L. G. Cleland, Biochim. Biophys. Acta 597, 418 (1980). 24 E. Ralston, L. M. Hjelmeland, R. D. Klausner, J. N. Weinstein, and R. Blumenthal, Biochim. Biophys. Acta 649, 133 (1981). [1] liposome coupling of fab0 fragments 15 measured (using excitation/emission wavelengths of 490/520 nm and excitation/emission slit widths of 5 nm) after dilution into coupling buffer vs coupling buffer containing 1% (v/v) Triton X-100.

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