Current Protocols Essential Laboratory Techniques by Sean R. Gallagher, Emily A. Wiley

By Sean R. Gallagher, Emily A. Wiley

The most recent identify from the acclaimed Current Protocols sequence, Current Protocols crucial Laboratory strategies, 2e presents the recent researcher with the abilities and figuring out of the basic laboratory techniques essential to run winning experiments, clear up difficulties, and turn into a effective member of the trendy existence technology laboratory.  From overlaying the elemental abilities similar to dimension, guidance of reagents and use of uncomplicated instrumentation to the extra complicated suggestions comparable to blotting, chromatography and real-time PCR, this booklet will function a realistic reference handbook for any lifestyles technological know-how researcher.  Written via a mixture of exceptional investigators and remarkable school, Current Protocols crucial Laboratory recommendations, 2e is the cornerstone on which the start scientist can boost the talents for a successful research occupation.

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Check the accuracy of the balance periodically with reference weights, and calibrate as necessary. Keep calibration masses in a safe, dry place. It is recommended that the balance be “locked” when not in use. Please refer to the manufacturer’s instruction manual for more detail. Improper use or maintenance of the balance can destroy the ability of the balance to measure mass accurately. Placing too large a mass on the platform or dropping items on the balance too roughly can deform the strain gauges in the load cell.

Drying In general, volumetric glassware can be dried in an oven at 105◦ to 110◦ C for an hour or more to ensure complete removal of water. If organic solvents have been used to rinse the glassware, it should be left in a fume hood to allow evaporation to occur. If necessary, the glassware may then be put in the oven for a shorter time to remove any traces of water. In the past there was concern about oven drying affecting the volumes of volumetric flasks or pipets, but a published study suggests that this is not a significant problem (Burfield and Hefter, 1987).

If the same object is weighed on the moon, its mass will remain constant but its weight will be ∼ 16 its weight on earth. It is important to understand these definitions for two very practical reasons. First of all, they contrast with practical and commercial usage (Barry, 1995). Generally, when a person tells somebody his weight in pounds or kilograms, he is really describing his mass. 11 Copyright C 2008 John Wiley & Sons, Inc. 2 Differences Between Mass and Weighta,b 1 Mass Weight Definition The quantity of material in a body The force by which a mass is attracted to the center of the earth by gravity Units, Systeme Internationale milligram (mg) gram (g) kilogram (kg) dyne (dyn) newton (N) kilonewton (kN) kilogram-force (kgf) Units, avoirdupois ounce (oz) pound (lb) ton ounce-force (ozf) pound-force (lbf) poundal ton-force a Sources: Scale Manufacturers Association (1981); Barry (1995).

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