Ciba Foundation Symposium 164 - Interactions Among Cell by CIBA Foundation Symposium

By CIBA Foundation Symposium

A panel of the world over popular specialists current papers on mobile signalling--an region within which there was contemporary vital advances. insurance contains the inositol 1, four, 5-triphosphate receptor, signal-induced phospholipid degradation cascade and protein kinase C activation, cyclic AMP interactions in sustained mobile reaction, the acetylcholine receptor and masses extra.

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J Biol Chem 265:18707-18712 Ryu SH, Cho KS, Lee K-Y, Suh P-G, Rhee SG 1987a Purification and characterization of two immunologicallydistinct phosphoinositide-specificphospholipase C from bovine brain. J Biol Chem 262:12511-12518 Ryu SH, Suh P-G, Cho KS, Lee K-Y, Rhee SG 1987b Bovine brain cytosol contains three immunologically distinct forms of inositol phospholipid-specific phospholipase C. Proc Natl Acad Sci USA 84:6649-6653 Smrcka AV, Hepler JR, Brown KO, Sternweis PC 1991 Regulation of a polyphosphoinositide-specificphospholipase C activity by purified G,.

These results suggest that InsP3-induced Ca2+ release is regulated by protein kinase A and also slightly by Ca2 /calmodulin-dependent protein kinase I1 and protein kinase C. Cross-linkage of the InsP3 receptor was done to investigate its subunit composition. Agarose-PAGE analysis after cross-linkage of the purified receptor revealed four distinct bands of M, 320000, 650000, 1000000 and 1 250000 (Fig. 1) (Maeda et a1 1991). No higher molecular weight cross-linked products were observed at any of the concentrations of cross-linker used.

A slight difference in Ca2+-pumping activity was detected between the two cell types. In each experiment the level of 45Ca2+ accumulated after 17 rnin in the membrane fractions from cells expressing the receptor was about 85% of that in the fractions from cells containing only the expression vector. channel that is opened by InsP3 (Fig. 5 ) . The channels show several conductance states. Addition of ATP in the presence of InsP, generated large conductance currents, probably resulting from a change in the full open conductance level or a shift to a greater conductance state.

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