By Harry J. Gilbert (Eds.)
Cellulase refers to a category of enzymes produced mainly by way of fungi, micro organism, and protozoans that catalyze cellulolysis. This quantity of Methods in Enzymology comprehensively covers this subject. With a global board of authors, this quantity covers matters such as ''The DNSA lowering assay for measuring cellulases,'' ''Measuring processivity'' and ''In situ cellulose detection with carbohydrate-binding modules.''
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The substrate is pretreated corn stover, which was washed, ground, and sieved (WGS-PCS). The substrate is mixed with a fluorescence enhancer (Calcofluor White, FB28) and the enzymatic hydrolysis of the substrate is monitored as a decrease in fluorescence emission intensity. FB28 is a compound that binds to cellulose and, when cellulose is hydrolyzed, FB28 is released. , 2001). Consequently, the emission response decreases upon hydrolysis of cellulose. , 2012) at 50 C with a goal of 80% conversion of cellulose to glucose, in alignment with possible commercial process timelines, and efficiency targets for hydrolysis.
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Cellazyme C, Cellazyme T, and Beta-Glucazyme) are useful for assaying enzyme activity in solutions. 2. 5 ml of appropriately diluted and buffered enzyme solution to the bottom of a 16 Â 120 mm glass test tube and equilibrate at 40 C for 5 min. Using forceps, add a Cellazyme C tablet (or Cellazyme T or Beta-Glucazyme tablet) and allow the reaction to proceed for 10 min (do not stir the tube contents). The tablet rapidly hydrates and absorbs the buffered enzyme solution. After 10 min add 10 ml of 2% (w/v) Trizma Base (pH $ 9) or 2% trisodium phosphate (pH 11) and vigorously stir the tube contents for 10 s.