Carotenoids Part A: Chemistry, Separation, Quantitation, and by Lester Packer (Eds.)

By Lester Packer (Eds.)

This quantity and its significant other, quantity 214 of Methods in Enzymology, current a complete, cutting-edge compilation of the molecular and mobile technique wanted for pursuing learn with carotenoids

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Ookubo, Nippon Suisan Gakkaishi 48, 653 (1982). [4] S u r f a c e - E n h a n c e d of Photosynthetic Raman Scattering Spectroscopy Membranes and Complexes By M I C H A E L SEIBERT, R A F A E L PICOREL, J A E - H O K I M , and THERESE M . COTTON Introduction Surface-enhanced Raman scattering (SERS) results when ion complexes, molecules, or chromophores adsorbed onto or near roughened silver, gold, or copper substrates are excited with laser light. The effect was first reported for pyridine on anodized silver electrodes ~-3 where an enM.

26 CHEMISTRY: SYNTHESIS, PROPERTIES, CHARACTERIZATION [3] 2 I | I 20 30 40 I 50 min Fie. 2. HPLC separation of two stereoisomers of phoenicoxanthin obtained from P. brevipes. 0 ml/min; detection, 450nm. Peak 1, (3R)phoenicoxanthin; peak 2, (3S)-phoenicoxanthin. 3 40 50 60 70 min F1G. 3. HPLC separation of three stereoisomers of astaxanthin obtained from P. brevipes. Conditions as in Fig. 2. Peak 1, (3R,3'R)-astaxanthin; peak 2, (3R,3'S, meso)-astaxanthin; peak 3, (3S,3'S)-astaxanthin. [3] STRUCTURE AND CHARACTERIZATION OF CAROTENOIDS 27 Instruments Melting points are m e a s u r e d o n a Y a n a g i m o t o (Kyoto, J a p a n ) micromelting p o i n t apparatus.

Krinsky, M. M. MathewsRoth, and R. F. ), Proc. 8th Int. Syrup. Carotenoids, p. 337. Plenum, New York, 1989. 213 Copyright© 1992byAcademicPress,Inc. Allrightsofreproductionin anyformreserved. 42 CHEMISTRY: SYNTHESIS, PROPERTIES, CHARACTERIZATION [5] biomolecular structure on surfaces. The membrane studies suggest that SERRS can provide qualitative information about the location and orientation of chromophores within the membrane. , charge-coupled devices), which have very low dark counts and can be used to integrate signals for long time periods.

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