Biomembranes Part A by Sidney Fleischer, Lester Packer (Eds.)

By Sidney Fleischer, Lester Packer (Eds.)

The significantly acclaimed laboratory general, Methods in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. due to the fact that 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct at the present time - actually an important ebook for researchers in all fields of existence sciences

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4) after osmotic shock. The samples after isolation from the gradient had good morphology (cf. Fig. 8) but were damaged by osmotic shock after dilution with distilled water and sedimentation, prior to fixation for electron microscopy (cf. Fig. 8). The length of the bar denotes 1 #m. [2] SUBCELLULAR FRACTIONATION OF RAT LIVER 31 TABLE II LIPID CONTENT OF LIVER CELL FRACTIONSa Fraction Phospholipid protein (#moles/mg) Cholesterol protein (vmoles/mg) Cholesterol phospholipid (molar ratio) Plasma membrane Nuclei Golgi complex Golgi membranesb Mitochondria Rough microsomesc 0.

J. 59, 438 (1955). S. Fleischer and B. Fleischer, this series Vol. 10, p. 406 (cf. pp. 427-428 for cytochrome c reductase assays). '~ S. Fleischer, B. Fleischer, A. Azzi, and Britton Chance, Biochim. Biophys. Acta 225, 194 (1971). J. R. Tata, this volume [24]. ° A. Trouet, this volume [31]. P. Baudhuin, H. B. Beaufay, Y. Rahman-Li, O. Z. Sellinger, R. Wattiaux, P. Jacques, and C. de Duve, Biochem. J . 92, 179 (1964). q F. Leighton, B. Poole, P. B. Lazarow, and C. de Duve, J. Cell Biol. 41,521 (1969).

Walton, and S. Fleischer, Bioenergetics 1, 247 (1970). L. M. G. van Golde, B. Fleischer, and S. Fleischer, Biochim. Biophys. Acta 249, 318 (1971). H. G. Hers, J. Berthet, L. Berthet, and C. de Duve, Bull. Soc. Chirn. Biol. 33, 21 (1951). w IN. G. Anderson and J. G. Green, in "Enzyme Cytology" (D. B. ), p. 475. Academic Press, New York, 1967. x O. H. Lowry, this series, Vol. 4, p. 366. 26 MULTIPLE FRACTIONS FROM A SINGLE TISSUE [2] FIG. 3. Electron micrograph of a section of liver illustrating the appearance of cell organelles in the cell: nuclei, N, rough endoplasmic reticulum, RER; mitochondria, M, Golgi complex, G; plasma membrane, PM; peroxisomes, P, and lysosomes, L.

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